RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism.
Identifieur interne : 003834 ( Main/Exploration ); précédent : 003833; suivant : 003835RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism.
Auteurs : Heather D. Coleman [Canada] ; Ji-Young Park ; Ramesh Nair ; Clint Chapple ; Shawn D. MansfieldSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2008.
Descripteurs français
- KwdFr :
- Interférence par ARN (MeSH), Lignine (composition chimique), Lignine (métabolisme), Mixed function oxygenases (génétique), Mixed function oxygenases (métabolisme), Populus (enzymologie), Populus (génétique), Régulation de l'expression des gènes codant pour des enzymes (génétique), Solubilité (MeSH), Structure moléculaire (MeSH), Transcription génétique (génétique).
- MESH :
- composition chimique : Lignine.
- enzymologie : Populus.
- génétique : Mixed function oxygenases, Populus, Régulation de l'expression des gènes codant pour des enzymes, Transcription génétique.
- métabolisme : Lignine, Mixed function oxygenases.
- Interférence par ARN, Solubilité, Structure moléculaire.
English descriptors
- KwdEn :
- Gene Expression Regulation, Enzymologic (genetics), Lignin (chemistry), Lignin (metabolism), Mixed Function Oxygenases (genetics), Mixed Function Oxygenases (metabolism), Molecular Structure (MeSH), Populus (enzymology), Populus (genetics), RNA Interference (MeSH), Solubility (MeSH), Transcription, Genetic (genetics).
- MESH :
- chemical , chemistry : Lignin.
- enzymology : Populus.
- genetics : Gene Expression Regulation, Enzymologic, Mixed Function Oxygenases, Populus, Transcription, Genetic.
- chemical , metabolism : Lignin, Mixed Function Oxygenases.
- Molecular Structure, RNA Interference, Solubility.
Abstract
p-Coumaroyl-CoA 3'-hydroxylase (C3'H) is a cytochrome P450-dependent monooxygenase that catalyzes the 3'-hydroxylation of p-coumaroyl shikimate and p-coumaroyl quinate. We used RNA interference to generate transgenic hybrid poplar suppressed in C3'H expression and analyzed them with respect to transcript abundance, cell wall structure and chemical composition, and soluble metabolite levels. RT-PCR expression profiles confirmed the down-regulation of C3'H in a number of lines, which generally correlated very well with reduced total cell wall lignin content. The most strongly repressed line was chosen for further analysis and compared with the wild-type trees. In-depth characterization revealed that along with the significant decrease in total lignin content, a significant shift in lignin monomer composition was observed, favoring the generation of p-hydroxyphenyl units at the expense of guaiacyl units while the proportion of syringyl moieties remained constant. Suppression of C3'H also resulted in the accumulation of substantial pools of 1-O-p-coumaroyl-beta-d-glucoside and other phenylpropanoid glycosides, and p-coumaroyl shikimate, providing further insight into the role of C3'H in the lignin biosynthetic pathway. The data presented indicate that when down-regulated, C3'H becomes a rate-limiting step in lignin biosynthesis and further support the involvement of hydroxycinnamic acid shikimate esters in the lignin biosynthetic pathway.
DOI: 10.1073/pnas.0706537105
PubMed: 18316744
PubMed Central: PMC2393750
Affiliations:
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Coleman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, Canada V6T 1Z4.</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Park, Ji Young" sort="Park, Ji Young" uniqKey="Park J" first="Ji-Young" last="Park">Ji-Young Park</name></author><author><name sortKey="Nair, Ramesh" sort="Nair, Ramesh" uniqKey="Nair R" first="Ramesh" last="Nair">Ramesh Nair</name></author><author><name sortKey="Chapple, Clint" sort="Chapple, Clint" uniqKey="Chapple C" first="Clint" last="Chapple">Clint Chapple</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18316744</idno><idno type="pmid">18316744</idno><idno type="doi">10.1073/pnas.0706537105</idno><idno type="pmc">PMC2393750</idno><idno type="wicri:Area/Main/Corpus">003941</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003941</idno><idno type="wicri:Area/Main/Curation">003941</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003941</idno><idno type="wicri:Area/Main/Exploration">003941</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism.</title><author><name sortKey="Coleman, Heather D" sort="Coleman, Heather D" uniqKey="Coleman H" first="Heather D" last="Coleman">Heather D. Coleman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, Canada V6T 1Z4.</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Park, Ji Young" sort="Park, Ji Young" uniqKey="Park J" first="Ji-Young" last="Park">Ji-Young Park</name></author><author><name sortKey="Nair, Ramesh" sort="Nair, Ramesh" uniqKey="Nair R" first="Ramesh" last="Nair">Ramesh Nair</name></author><author><name sortKey="Chapple, Clint" sort="Chapple, Clint" uniqKey="Chapple C" first="Clint" last="Chapple">Clint Chapple</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Enzymologic (genetics)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Mixed Function Oxygenases (genetics)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Molecular Structure (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>RNA Interference (MeSH)</term><term>Solubility (MeSH)</term><term>Transcription, Genetic (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Interférence par ARN (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Mixed function oxygenases (génétique)</term><term>Mixed function oxygenases (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Régulation de l'expression des gènes codant pour des enzymes (génétique)</term><term>Solubilité (MeSH)</term><term>Structure moléculaire (MeSH)</term><term>Transcription génétique (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Enzymologic</term><term>Mixed Function Oxygenases</term><term>Populus</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mixed function oxygenases</term><term>Populus</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Transcription génétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Mixed function oxygenases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Structure</term><term>RNA Interference</term><term>Solubility</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interférence par ARN</term><term>Solubilité</term><term>Structure moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">p-Coumaroyl-CoA 3'-hydroxylase (C3'H) is a cytochrome P450-dependent monooxygenase that catalyzes the 3'-hydroxylation of p-coumaroyl shikimate and p-coumaroyl quinate. We used RNA interference to generate transgenic hybrid poplar suppressed in C3'H expression and analyzed them with respect to transcript abundance, cell wall structure and chemical composition, and soluble metabolite levels. RT-PCR expression profiles confirmed the down-regulation of C3'H in a number of lines, which generally correlated very well with reduced total cell wall lignin content. The most strongly repressed line was chosen for further analysis and compared with the wild-type trees. In-depth characterization revealed that along with the significant decrease in total lignin content, a significant shift in lignin monomer composition was observed, favoring the generation of p-hydroxyphenyl units at the expense of guaiacyl units while the proportion of syringyl moieties remained constant. Suppression of C3'H also resulted in the accumulation of substantial pools of 1-O-p-coumaroyl-beta-d-glucoside and other phenylpropanoid glycosides, and p-coumaroyl shikimate, providing further insight into the role of C3'H in the lignin biosynthetic pathway. The data presented indicate that when down-regulated, C3'H becomes a rate-limiting step in lignin biosynthesis and further support the involvement of hydroxycinnamic acid shikimate esters in the lignin biosynthetic pathway.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18316744</PMID><DateCompleted><Year>2008</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>105</Volume><Issue>11</Issue><PubDate><Year>2008</Year><Month>Mar</Month><Day>18</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism.</ArticleTitle><Pagination><MedlinePgn>4501-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.0706537105</ELocationID><Abstract><AbstractText>p-Coumaroyl-CoA 3'-hydroxylase (C3'H) is a cytochrome P450-dependent monooxygenase that catalyzes the 3'-hydroxylation of p-coumaroyl shikimate and p-coumaroyl quinate. We used RNA interference to generate transgenic hybrid poplar suppressed in C3'H expression and analyzed them with respect to transcript abundance, cell wall structure and chemical composition, and soluble metabolite levels. RT-PCR expression profiles confirmed the down-regulation of C3'H in a number of lines, which generally correlated very well with reduced total cell wall lignin content. The most strongly repressed line was chosen for further analysis and compared with the wild-type trees. In-depth characterization revealed that along with the significant decrease in total lignin content, a significant shift in lignin monomer composition was observed, favoring the generation of p-hydroxyphenyl units at the expense of guaiacyl units while the proportion of syringyl moieties remained constant. Suppression of C3'H also resulted in the accumulation of substantial pools of 1-O-p-coumaroyl-beta-d-glucoside and other phenylpropanoid glycosides, and p-coumaroyl shikimate, providing further insight into the role of C3'H in the lignin biosynthetic pathway. The data presented indicate that when down-regulated, C3'H becomes a rate-limiting step in lignin biosynthesis and further support the involvement of hydroxycinnamic acid shikimate esters in the lignin biosynthetic pathway.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Coleman</LastName><ForeName>Heather D</ForeName><Initials>HD</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, Canada V6T 1Z4.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Ji-Young</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Nair</LastName><ForeName>Ramesh</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chapple</LastName><ForeName>Clint</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>03</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.17.-</RegistryNumber><NameOfSubstance 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Mansfield</name><name sortKey="Nair, Ramesh" sort="Nair, Ramesh" uniqKey="Nair R" first="Ramesh" last="Nair">Ramesh Nair</name><name sortKey="Park, Ji Young" sort="Park, Ji Young" uniqKey="Park J" first="Ji-Young" last="Park">Ji-Young Park</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Coleman, Heather D" sort="Coleman, Heather D" uniqKey="Coleman H" first="Heather D" last="Coleman">Heather D. Coleman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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